Backbone 1H, 15N and 13C resonance assignments of the 27kDa fluorescent protein mCherry
| Author | |
| Abstract |
mCherry is one of the most successfully applied monomeric red fluorescent proteins (RFPs) for in vivo and in vitro imaging. However, questions pertaining to the photostability of the RFPs remain and rational further engineering of their photostability requires information about the fluorescence quenching mechanism in solution. To this end, NMR spectroscopic investigations might be helpful, and we present the near-complete backbone NMR chemical shift assignment to aid in this pursuit. |
| Year of Publication |
2023
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| Date Published |
2024-02
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| Journal Title |
Biomolecular NMR Assignments
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| Volume |
17
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| Issue |
2
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| Start Page or Article ID (correct) |
243-247
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| ISSN Number |
1874-2718, 1874-270X
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| DOI | |
| Download citation | |
| JILA PI | |
| Associated Institutes | |
Journal Article
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| JILA Topics | |
| Publication Status |
The Physics Frontiers Centers (PFC) program supports university-based centers and institutes where the collective efforts of a larger group of individuals can enable transformational advances in the most promising research areas. The program is designed to foster major breakthroughs at the intellectual frontiers of physics by providing needed resources such as combinations of talents, skills, disciplines, and/or specialized infrastructure, not usually available to individual investigators or small groups, in an environment in which the collective efforts of the larger group can be shown to be seminal to promoting significant progress in the science and the education of students. PFCs also include creative, substantive activities aimed at enhancing education, broadening participation of traditionally underrepresented groups, and outreach to the scientific community and general public.