Conformational dynamics of mCherry variants: a link between sidechain motions and fluorescence brightness

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Abstract

The 3-fold higher brightness of the recently developed mCherry-XL red fluorescent protein (FP) compared to its progenitor, mCherry, is due to a significant decrease in the nonradiative decay rate underlying its increased fluorescence quantum yield. To examine the structural and dynamic role of the four mutations that distinguish the two FPs and closely related variants, we employed microsecond time scale, all-atom molecular dynamics simulations.

Year of Publication
2022
Date Published
2022-12
Journal Title
The Journal of Physical Chemistry B
Volume
127
Issue
1
Start Page or Article ID (correct)
52–61
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