Single Molecule Biophysics: Microscopy, Kinetics and Thermodynamics

Single-Molecule FRET Kinetics of the Mn2+ Riboswitch: Evidence for Allosteric Mg2+ Control of “Induced-Fit” vs “Conformational Selection” Folding Pathways
[1]
H.-L. . Sung and D. J. Nesbitt, The Journal of Physical Chemistry B 123, (2019).
Novel Heat-Promoted Folding Dynamics of the yybP-ykoY Manganese Riboswitch: Kinetic and Thermodynamic Studies at the Single-Molecule Level
[1]
H.-L. . Sung and D. J. Nesbitt, The Journal of Physical Chemistry B 123, (2019).
DNA Hairpin Hybridization under Extreme Pressures: A Single-Molecule FRET Study
[1]
H.-L. . Sung and D. J. Nesbitt, The Journal of Physical Chemistry B 124, (2020).
Single-Molecule Kinetic Studies of DNA Hybridization Under Extreme Pressures
[1]
H.-L. . Sung and D. J. Nesbitt, Physical Chemistry Chemical Physics 22, (2020).
Chirality-Dependent Amino Acid Modulation of RNA Folding
[1]
D. A. Nicholson, A. . Sengupta, and D. J. Nesbitt, The Journal of Physical Chemistry B 124, (2020).
Smaller molecules crowd better: Crowder size dependence revealed by single-molecule FRET studies and depletion force modeling analysis
[1]
H.-L. . Sung, A. . Sengupta, and D. J. Nesbitt, The Journal of Chemical Physics 154, (2021).
Pushing Camera-Based Single-Molecule Kinetic Measurements to the Frame Acquisition Limit with Stroboscopic smFRET
[1]
D. A. Nicholson and D. J. Nesbitt, The Journal of Physical Chemistry B (2021).
Sequential folding of the nickel/cobalt riboswitch is facilitated by a conformational intermediate: Insights from single-molecule kinetics and thermodynamics
[1]
H.-L. . Sung and D. J. Nesbitt, The Journal of Physical Chemistry B 124, (2020).
Correction: High pressure single-molecule FRET studies of the lysine riboswitch: cationic and osmolytic effects on pressure induced denaturation
[1]
H.-L. . Sung and D. J. Nesbitt, Phys. Chem. Chem. Phys. 22, (2020).
Measuring Excess Heat Capacities of Deoxyribonucleic Acid (DNA) Folding at the Single-Molecule Level
[1]
D. A. Nicholson, B. . Jia, and D. J. Nesbitt, The Journal of Physical Chemistry B 125, (2021).
The Effects of Molecular Crowders on a Single Molecule Nucleic Acid Folding: Temperature Dependent Studies Reveal True Crowding vs. Enthalpic Interactions
[1]
D. J. Nesbitt, The Journal of Physical Chemistry B 125, (2021).
Lysine-Dependent Entropy Effects in the B. subtilis Lysine Riboswitch: Insights from Single-Molecule Thermodynamic Studies
[1]
A. . Menendez and D. J. Nesbitt, The Journal of Physical Chemistry B 126, (2022).
Synergism in the Molecular Crowding of Ligand-Induced Riboswitch Folding: Kinetic/Thermodynamic Insights from Single-Molecule Spectroscopy
[1]
H.-L. . Sung and D. J. Nesbitt, The Journal of Physical Chemistry B 126, (2022).
Ligand-Dependent Volumetric Characterization of Manganese Riboswitch Folding: A High-Pressure Single-Molecule Kinetic Study
[1]
H.-L. . Sung and D. J. Nesbitt, Journal of Physical Chemistry B 126, (2022).
Ionic Cooperativity between Lysine and Potassium in the Lysine Riboswitch: Single-Molecule Kinetic and Thermodynamic Studies
[1]
A. . Menendez and D. J. Nesbitt, The Journal of Physical Chemistry B 127, (2023).
Kinetic and Thermodynamic Control of G-Quadruplex Polymorphism by Na<sup>+</sup> and K<sup>+</sup> Cations
[1]
D. A. Nicholson and D. J. Nesbitt, The Journal of Physical Chemistry B 127, (2023).
Thermodynamic compensation in extreme thermal environments: single molecule dependent kinetic studies of B. subtilis and T.maritima lysine riboswitches
[1]
D. J. Nesbitt and A. . Menendez, Biophysical Journal (2024).