@article{4273,
  keywords = {dentin-enamel junction, Raman spectroscopy},
  author = {Ping Wang and Evan Anderson and Eric Muller and Fuhua Gao and Yisi Zhong and Markus Raschke},
  title = {Hyper-spectral Raman imaging correlating chemical substitution and crystallinity in biogenic hydroxyapatite: Dentin and enamel in normal and hypoplastic human teeth},
  abstract = {<p>Micro-Raman imaging and spectroscopy has become an established technique<br /> for the characterization of biogenic hydroxyapatite as, for example, the primary<br /> constituent of human teeth. However, few studies have yet gone beyond a<br /> qualitative analysis of the Raman response providing only limited insight into<br /> spatial heterogeneity of composition, structure, and degree of crystallinity.<br /> Here, we show how correlative electron microprobe and extended<br /> hyperspectral Raman imaging with high spatial and spectral resolution, with<br /> peak position and linewidth analysis, and from the μm to mm scale, provides<br /> insight into structural characteristics in dentin and enamel. From comparison<br /> of healthy and hypoplastic teeth as a representative tooth disease example, we<br /> determine variations in degree of crystallinity, both locally across the dentin\textendash<br /> enamel junction, and with distinct long-range spatial variations. We identify<br /> a correlation of spectral peak position and linewidth as a measure of crystal<br /> lattice disorder across tubules, dentin, dentin\textendashenamel junction, and enamel.<br /> This correlative Raman imaging and analysis approach may help to provide a<br /> better understanding of apatite geochemistry and biomineralization.<br /> \&nbsp;</p>},
  year = {2018},
  journal = {Journal of Raman Spectroscopy},
  volume = {49},
  month = {2018-07},
  url = {https://onlinelibrary.wiley.com/doi/epdf/10.1002/jrs.5419},
  doi = {10.1002/jrs.v49.910.1002/jrs.5419},
}