@article{12335, keywords = {Physical and Theoretical Chemistry}, author = {Wyatt Zagorec-Marks and Madison Foreman and J. Mathias Weber}, title = {Tag-Free, Temperature Dependent Infrared Spectra of the GFP Chromophore: Revisiting the Question of Isomerism}, abstract = {We report infrared spectra of a model chromophore of green fluorescent protein, prepared in an ion trap at temperatures ranging from 30 K to room temperature. We compare the changes in the infrared spectrum with predicted infrared spectra for the Z and E isomers of this molecule, and we confirm that the molecule exists as the Z isomer at low temperatures. We revisit the question whether or not it can thermally isomerize in the temperature range of this experiment, and we find no evidence for isomerization.}, year = {2020}, journal = {The Journal of Physical Chemistry A}, volume = {124}, pages = {7827-7831}, month = {2020-08}, publisher = {American Chemical Society (ACS)}, issn = {1089-5639, 1520-5215}, doi = {10.1021/acs.jpca.0c07172}, }