@article{12134,
  author = {Hao Yu and David Jacobson and Hao Luo and Thomas Perkins},
  title = {Quantifying the native energetics stabilizing bacteriorhodopsin by single-molecule force spectroscopy},
  abstract = {We quantified the equilibrium (un)folding free energy ΔG0 of an eight-amino-acid region starting from the fully folded state of the model membrane-protein bacteriorhodopsin using single-molecule force spectroscopy. Analysis of equilibrium and nonequilibrium data yielded consistent, high-precision determinations of ΔG0 via multiple techniques (force-dependent kinetics, Crooks fluctuation theorem, and inverse Boltzmann analysis). We also deduced the full 1D projection of the free-energy landscape in this region. Importantly, ΔG0 was determined in bacteriorhodopsin’s native bilayer, an advance over traditional results obtained by chemical denaturation in nonphysiological detergent micelles.
},
  year = {2020},
  journal = {Physical Review Letters},
  volume = {125},
  chapter = {068102},
  pages = {068102},
  month = {2020-08},
  publisher = {American Physical Society},
  url = {https://link.aps.org/doi/10.1103/PhysRevLett.125.068102},
  doi = {10.1103/PhysRevLett.125.068102},
}